Peplo · Reconstitution calculator

TB-500

Synthetic fragment of Thymosin Beta-4; studied for actin regulation, soft-tissue repair, and angiogenesis.

healing

Reconstitution calculator

Vial Amount

mg

The total peptide in the vial — the mg number on the label (e.g. 5 mg). This is your whole supply, not one dose.

Bacteriostatic Water

mL

The sterile water you add to dissolve the powder (this is called reconstituting). More water makes small doses easier to measure.

Desired Dose

mcg

How much you take in one injection. 1 mg = 1000 mcg.

Syringe Size

Draw to 120 units

1.20 mL on a U-100 insulin syringe

A starting reference, not medical advice — see how we source this.

Half-life: ~48 h
Route: SubQ
Storage: Refrigerated 2-8°C; use within ~30 days (~30 days)
Citations: 12 referenced

US regulatory status

Not approved by the FDA for human use; sold and used as a research compound.

What is TB-500?

TB-500 is a synthetic fragment of the naturally occurring protein Thymosin Beta-4. It has been studied in preclinical models for its role in actin regulation, cell migration, and tissue repair.

How TB-500 works

Binds actin and promotes cell migration, vascularization, and tissue remodelling at sites of injury.

What the research says

Research on TB-500 and Thymosin Beta-4 has examined angiogenesis, wound healing, and cardiac and soft-tissue repair, predominantly in animal and in-vitro models.

Evidence limitations

Human clinical evidence is limited; primary sources are linked below.

Safety & use boundary

TB-500 is a research compound and is not approved for human use. Educational information only — not medical advice.

Published protocols

Dosing figures reported in the cited research. Educational reference, not a recommendation.

recovery
2000–5000 mcg, twice weekly
2005 study
injury-repair
5000–7500 mcg, twice weekly
2005 study
longevity
1000–2000 mcg, once weekly
2005 study

How to reconstitute TB-500

New to this? Here's the whole process, start to finish. Educational only — not medical advice.

What you'll need

The TB-500 vial — a small glass vial of white, freeze-dried powder
Bacteriostatic water — the liquid you mix in (not plain sterile or tap water)
U-100 insulin syringes — the small syringes marked in units
Alcohol swabs to wipe the vial tops before you touch them with a needle
A spot in the fridge to keep it once it's mixed

Step by step

Wipe everything down
Swab the rubber top of both the peptide vial and the bacteriostatic water with an alcohol wipe. Clean tops are what keep the mixed solution good for weeks instead of days.
Draw the water
Use the calculator above to see how much bacteriostatic water to add for your vial size. Pull exactly that amount out of the water vial with a syringe.
Add it slowly
Push the needle into the peptide vial and let the water run gently down the inside glass wall — don't blast it straight onto the powder.
Let it dissolve
Swirl the vial gently or just let it sit. The powder goes into solution on its own in a minute or two. Never shake it hard.
Read your draw
Enter your vial size, the water you added, and your dose in the calculator. It shows the exact units to pull into your syringe — you never do the math yourself.
Store it cold
Cap it and keep it in the fridge. TB-500 stays good for about 30 days once it's mixed.

Reading your dose

The calculator gives you a number like “Draw to 20 units.” Units are just the numbered marks on a U-100 insulin syringe (100 units fill 1 mL). “20 units” means pull the plunger back to the 20 mark. You don't convert milligrams to micrograms in your head — that's the whole point of the calculator. If you're brand new to injecting, learn proper technique and site rotation from a qualified clinician or nurse before your first dose; this page covers the mixing and measuring, not the injection itself.

Storing it

Before mixing: Refrigerated; can ship at ambient briefly Once reconstituted: Refrigerated 2-8°C; use within ~30 days (about 30 days). Don't freeze a reconstituted vial.

What “cycling” means

Many peptides are used in “cycles” — a stretch of daily or weekly use (“on”), then a break (“off”), rather than continuously. Cycling gives the body a rest and is a common pattern in the research. Any specific on/off lengths for a compound live in its published protocols above, drawn from the cited studies — this site shows you what the research used, it doesn't recommend a schedule for you.

Common first-timer mistakes

Shaking the vial to mix it
Swirl it gently or just let it sit. Shaking can damage delicate peptides — you want the powder to dissolve, not foam.
Using tap water or plain sterile water
Use bacteriostatic water. Plain sterile water has no preservative, so a mixed vial spoils in a day or two instead of lasting weeks.
Leaving a mixed vial on the counter
Keep reconstituted vials in the fridge. Heat and light break the peptide down faster.
Eyeballing the dose instead of measuring
Use the calculator and draw to the exact unit mark on the syringe. Guessing a tiny volume is where most measuring errors happen.
Reusing needles
Use a fresh syringe each time. A used needle is duller, hurts more, and is no longer sterile.
Mixing up mg and mcg
1 mg = 1000 mcg. The calculator keeps them straight so you don't have to — just enter your dose in the unit it asks for.

Words you'll see

Reconstitution: Mixing a freeze-dried peptide powder with liquid (bacteriostatic water) so it can be drawn into a syringe.
Bacteriostatic water: Sterile water with a tiny amount of benzyl alcohol that stops bacteria growing, so a mixed vial keeps for weeks. Not the same as plain sterile water.
U-100 insulin syringe: A small syringe marked in “units,” where 100 units = 1 mL. Peptide draws are measured in these units.
Subcutaneous (SubQ): An injection into the fatty layer just under the skin, rather than into muscle or a vein.
mcg vs mg: 1 mg = 1000 mcg. Doses are often written in micrograms (mcg); the calculator handles the conversion for you.
Lyophilized: Freeze-dried. The powder in the vial is the peptide with its water removed so it stays stable on the shelf.
Half-life: Roughly how long it takes your body to clear half of a dose — a rough sense of how long it stays active.

Common questions

What is TB-500?

Synthetic fragment of Thymosin Beta-4; studied for actin regulation, soft-tissue repair, and angiogenesis. Binds actin and promotes cell migration, vascularization, and tissue remodelling at sites of injury.

How is TB-500 reconstituted?

TB-500 ships as a lyophilised powder and is reconstituted with bacteriostatic water before use, then drawn on an insulin syringe. Use the calculator above to see the exact units to draw for your vial size and dose. This is a reference tool, not medical advice.

What is the half-life of TB-500?

Research reports an approximate half-life of 48 hours for TB-500. Half-life informs how frequently a compound is studied; it is not a dosing instruction.

How should TB-500 be stored?

Refrigerated; can ship at ambient briefly Once reconstituted: Refrigerated 2-8°C; use within ~30 days (about 30 days). This is general storage information, not medical advice.

What is TB-500 commonly studied alongside?

In the research literature TB-500 is often discussed together with bpc 157. Co-occurrence in studies is not an endorsement of any combination.

Related compounds

bpc 157

Keep your protocol, doses, and vials in one place.

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