Peplo · Reconstitution calculator

Testagen

Synthetic tetrapeptide (Lys-Glu-Asp-Gly; KEDG), an anterior-pituitary-derived bioregulator studied preclinically for endocrine modulation of the pituitary-gonadal axis.

longevity

Reconstitution calculator

Vial Amount

The total peptide in the vial — the mg number on the label (e.g. 5 mg). This is your whole supply, not one dose.

Bacteriostatic Water

The sterile water you add to dissolve the powder (this is called reconstituting). More water makes small doses easier to measure.

Desired Dose

mcg

How much you take in one injection. 1 mg = 1000 mcg.

Syringe Size

Draw to 2.8 units

0.03 mL on a U-100 insulin syringe

A starting reference, not medical advice — see how we source this.

Half-life: Not established
Route: SubQ
Storage: Refrigerate at 2-8 °C, protected from light; avoid freeze-thaw cycles. (~30 days)
Citations: 2 referenced

US regulatory status

Not an approved medication; it has been examined only in preclinical animal studies and is used as a research compound.

What is Testagen?

Testagen is a synthetic tetrapeptide (Lys-Glu-Asp-Gly, or KEDG) described as an anterior-pituitary-derived bioregulator and administered subcutaneously in research settings. It has been studied preclinically for endocrine modulation of the pituitary-gonadal axis.

How Testagen works

Belongs to a class of short bioregulatory peptides from anterior pituitary extracts; preclinical avian studies show it can normalize thyroid hormone levels in hypophysectomized subjects and modulate pituitary-gonadal signaling, hypothesized to act through gene-regulatory effects on hormone synthesis. No human clinical trials exist.

What the research says

Testagen belongs to a class of short bioregulatory peptides associated with anterior pituitary extracts, and preclinical avian studies have examined its effects on thyroid hormone levels and pituitary-gonadal signaling. The cited work, including studies of Lys-Glu-Asp-Gly and related peptides in hypophysectomized chickens and older birds, looked at immunity, hemostasis, and thyroid gland morphology and function. Researchers have hypothesized that any effects may involve gene-regulatory influence on hormone synthesis.

Evidence limitations

No human clinical trials exist, so human evidence is limited.

Safety & use boundary

Testagen is a research compound that has been examined only in preclinical animal studies and is not an approved medication. This page is educational information and is not medical advice.

Published protocols

Dosing figures reported in the cited research. Educational reference, not a recommendation.

longevity
100–300 mcg, once daily
2010 study

How to reconstitute Testagen

New to this? Here's the whole process, start to finish. Educational only — not medical advice.

What you'll need

The Testagen vial — a small glass vial of white, freeze-dried powder
Bacteriostatic water — the liquid you mix in (not plain sterile or tap water)
U-100 insulin syringes — the small syringes marked in units
Alcohol swabs to wipe the vial tops before you touch them with a needle
A spot in the fridge to keep it once it's mixed

Step by step

Wipe everything down
Swab the rubber top of both the peptide vial and the bacteriostatic water with an alcohol wipe. Clean tops are what keep the mixed solution good for weeks instead of days.
Draw the water
Use the calculator above to see how much bacteriostatic water to add for your vial size. Pull exactly that amount out of the water vial with a syringe.
Add it slowly
Push the needle into the peptide vial and let the water run gently down the inside glass wall — don't blast it straight onto the powder.
Let it dissolve
Swirl the vial gently or just let it sit. The powder goes into solution on its own in a minute or two. Never shake it hard.
Read your draw
Enter your vial size, the water you added, and your dose in the calculator. It shows the exact units to pull into your syringe — you never do the math yourself.
Store it cold
Cap it and keep it in the fridge. Testagen stays good for about 30 days once it's mixed.

Reading your dose

The calculator gives you a number like “Draw to 20 units.” Units are just the numbered marks on a U-100 insulin syringe (100 units fill 1 mL). “20 units” means pull the plunger back to the 20 mark. You don't convert milligrams to micrograms in your head — that's the whole point of the calculator. If you're brand new to injecting, learn proper technique and site rotation from a qualified clinician or nurse before your first dose; this page covers the mixing and measuring, not the injection itself.

Storing it

Before mixing: Lyophilized: freeze at -20 °C in dry, dark conditions; minimize moisture exposure. Once reconstituted: Refrigerate at 2-8 °C, protected from light; avoid freeze-thaw cycles. (about 30 days). Don't freeze a reconstituted vial.

What “cycling” means

Many peptides are used in “cycles” — a stretch of daily or weekly use (“on”), then a break (“off”), rather than continuously. Cycling gives the body a rest and is a common pattern in the research. Any specific on/off lengths for a compound live in its published protocols above, drawn from the cited studies — this site shows you what the research used, it doesn't recommend a schedule for you.

Common first-timer mistakes

Shaking the vial to mix it
Swirl it gently or just let it sit. Shaking can damage delicate peptides — you want the powder to dissolve, not foam.
Using tap water or plain sterile water
Use bacteriostatic water. Plain sterile water has no preservative, so a mixed vial spoils in a day or two instead of lasting weeks.
Leaving a mixed vial on the counter
Keep reconstituted vials in the fridge. Heat and light break the peptide down faster.
Eyeballing the dose instead of measuring
Use the calculator and draw to the exact unit mark on the syringe. Guessing a tiny volume is where most measuring errors happen.
Reusing needles
Use a fresh syringe each time. A used needle is duller, hurts more, and is no longer sterile.
Mixing up mg and mcg
1 mg = 1000 mcg. The calculator keeps them straight so you don't have to — just enter your dose in the unit it asks for.

Words you'll see

Reconstitution: Mixing a freeze-dried peptide powder with liquid (bacteriostatic water) so it can be drawn into a syringe.
Bacteriostatic water: Sterile water with a tiny amount of benzyl alcohol that stops bacteria growing, so a mixed vial keeps for weeks. Not the same as plain sterile water.
U-100 insulin syringe: A small syringe marked in “units,” where 100 units = 1 mL. Peptide draws are measured in these units.
Subcutaneous (SubQ): An injection into the fatty layer just under the skin, rather than into muscle or a vein.
mcg vs mg: 1 mg = 1000 mcg. Doses are often written in micrograms (mcg); the calculator handles the conversion for you.
Lyophilized: Freeze-dried. The powder in the vial is the peptide with its water removed so it stays stable on the shelf.
Half-life: Roughly how long it takes your body to clear half of a dose — a rough sense of how long it stays active.

Common questions

What is Testagen?

Synthetic tetrapeptide (Lys-Glu-Asp-Gly; KEDG), an anterior-pituitary-derived bioregulator studied preclinically for endocrine modulation of the pituitary-gonadal axis. Belongs to a class of short bioregulatory peptides from anterior pituitary extracts; preclinical avian studies show it can normalize thyroid hormone levels in hypophysectomized subjects and modulate pituitary-gonadal signaling, hypothesized to act through gene-regulatory effects on hormone synthesis. No human clinical trials exist.

How is Testagen reconstituted?

Testagen ships as a lyophilised powder and is reconstituted with bacteriostatic water before use, then drawn on an insulin syringe. Use the calculator above to see the exact units to draw for your vial size and dose. This is a reference tool, not medical advice.

How should Testagen be stored?

Lyophilized: freeze at -20 °C in dry, dark conditions; minimize moisture exposure. Once reconstituted: Refrigerate at 2-8 °C, protected from light; avoid freeze-thaw cycles. (about 30 days). This is general storage information, not medical advice.

Keep your protocol, doses, and vials in one place.

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