Peplo · Reconstitution calculator
Follistatin-344
A synthetic myostatin/activin inhibitor studied in gene-therapy trials for muscle growth and Becker muscular dystrophy, distinct from injectable recombinant peptide use.
growth-factorReconstitution calculator
The total peptide in the vial — the mg number on the label (e.g. 5 mg). This is your whole supply, not one dose.
The sterile water you add to dissolve the powder (this is called reconstituting). More water makes small doses easier to measure.
How much you take in one injection. 1 mg = 1000 mcg.
Draw to 3 units
0.03 mL on a U-100 insulin syringe
A starting reference, not medical advice — see how we source this.
- Half-life
- Not established
- Route
- IM
- Storage
- Refrigerate at 2-8°C after reconstitution with bacteriostatic water; never freeze reconstituted solution. (~28 days)
- Citations
- 2 referenced
US regulatory status
Not approved by the FDA for general use; investigated in trial settings as an experimental research compound.
What is Follistatin-344?
Follistatin-344 is a synthetic myostatin and activin inhibitor in the growth-factor category, studied primarily in gene-therapy trials for muscle growth and Becker muscular dystrophy. It is described as distinct from injectable recombinant peptide use, and it works by sequestering myostatin (GDF-8) and activin A to relieve a natural brake on muscle growth.
How Follistatin-344 works
Follistatin-344 binds and sequesters myostatin (GDF-8) and activin A, preventing them from engaging ActRIIB receptors on muscle cells. This blocks the Smad 2/3 signaling cascade that normally suppresses protein synthesis and satellite cell activation, removing a natural brake on muscle growth.
What the research says
The cited literature includes a study of follistatin gene delivery enhancing muscle growth and strength in nonhuman primates and a phase 1/2a follistatin gene-therapy trial for Becker muscular dystrophy. These investigations examined the compound in a gene-therapy context, exploring effects on muscle mass and function.
Evidence limitations
The work reflects early-stage and preclinical-to-early-clinical study rather than established treatment. Broader human evidence remains limited.
Safety & use boundary
Follistatin-344 is handled here as an experimental research compound investigated in trial settings, not as an approved therapy for general use. This information is educational and is not medical advice.
Published protocols
Dosing figures reported in the cited research. Educational reference, not a recommendation.
- 2009 study
recovery
50–100 mcg, training days
How to reconstitute Follistatin-344
New to this? Here's the whole process, start to finish. Educational only — not medical advice.
What you'll need
- The Follistatin-344 vial — a small glass vial of white, freeze-dried powder
- Bacteriostatic water — the liquid you mix in (not plain sterile or tap water)
- U-100 insulin syringes — the small syringes marked in units
- Alcohol swabs to wipe the vial tops before you touch them with a needle
- A spot in the fridge to keep it once it's mixed
Step by step
Wipe everything down
Swab the rubber top of both the peptide vial and the bacteriostatic water with an alcohol wipe. Clean tops are what keep the mixed solution good for weeks instead of days.
Draw the water
Use the calculator above to see how much bacteriostatic water to add for your vial size. Pull exactly that amount out of the water vial with a syringe.
Add it slowly
Push the needle into the peptide vial and let the water run gently down the inside glass wall — don't blast it straight onto the powder.
Let it dissolve
Swirl the vial gently or just let it sit. The powder goes into solution on its own in a minute or two. Never shake it hard.
Read your draw
Enter your vial size, the water you added, and your dose in the calculator. It shows the exact units to pull into your syringe — you never do the math yourself.
Store it cold
Cap it and keep it in the fridge. Follistatin-344 stays good for about 28 days once it's mixed.
Reading your dose
The calculator gives you a number like “Draw to 20 units.” Units are just the numbered marks on a U-100 insulin syringe (100 units fill 1 mL). “20 units” means pull the plunger back to the 20 mark. You don't convert milligrams to micrograms in your head — that's the whole point of the calculator. If you're brand new to injecting, learn proper technique and site rotation from a qualified clinician or nurse before your first dose; this page covers the mixing and measuring, not the injection itself.
Storing it
Before mixing: Store lyophilized powder frozen at -20°C, stable 1+ year; refrigerated (2-8°C) stable 1-3 months; room temperature only 2-3 weeks in emergencies. Once reconstituted: Refrigerate at 2-8°C after reconstitution with bacteriostatic water; never freeze reconstituted solution. (about 28 days). Don't freeze a reconstituted vial.
What “cycling” means
Many peptides are used in “cycles” — a stretch of daily or weekly use (“on”), then a break (“off”), rather than continuously. Cycling gives the body a rest and is a common pattern in the research. Any specific on/off lengths for a compound live in its published protocols above, drawn from the cited studies — this site shows you what the research used, it doesn't recommend a schedule for you.
Common first-timer mistakes
Shaking the vial to mix it
Swirl it gently or just let it sit. Shaking can damage delicate peptides — you want the powder to dissolve, not foam.
Using tap water or plain sterile water
Use bacteriostatic water. Plain sterile water has no preservative, so a mixed vial spoils in a day or two instead of lasting weeks.
Leaving a mixed vial on the counter
Keep reconstituted vials in the fridge. Heat and light break the peptide down faster.
Eyeballing the dose instead of measuring
Use the calculator and draw to the exact unit mark on the syringe. Guessing a tiny volume is where most measuring errors happen.
Reusing needles
Use a fresh syringe each time. A used needle is duller, hurts more, and is no longer sterile.
Mixing up mg and mcg
1 mg = 1000 mcg. The calculator keeps them straight so you don't have to — just enter your dose in the unit it asks for.
Words you'll see
- Reconstitution
- Mixing a freeze-dried peptide powder with liquid (bacteriostatic water) so it can be drawn into a syringe.
- Bacteriostatic water
- Sterile water with a tiny amount of benzyl alcohol that stops bacteria growing, so a mixed vial keeps for weeks. Not the same as plain sterile water.
- U-100 insulin syringe
- A small syringe marked in “units,” where 100 units = 1 mL. Peptide draws are measured in these units.
- Subcutaneous (SubQ)
- An injection into the fatty layer just under the skin, rather than into muscle or a vein.
- mcg vs mg
- 1 mg = 1000 mcg. Doses are often written in micrograms (mcg); the calculator handles the conversion for you.
- Lyophilized
- Freeze-dried. The powder in the vial is the peptide with its water removed so it stays stable on the shelf.
- Half-life
- Roughly how long it takes your body to clear half of a dose — a rough sense of how long it stays active.
Common questions
What is Follistatin-344?
A synthetic myostatin/activin inhibitor studied in gene-therapy trials for muscle growth and Becker muscular dystrophy, distinct from injectable recombinant peptide use. Follistatin-344 binds and sequesters myostatin (GDF-8) and activin A, preventing them from engaging ActRIIB receptors on muscle cells. This blocks the Smad 2/3 signaling cascade that normally suppresses protein synthesis and satellite cell activation, removing a natural brake on muscle growth.
How is Follistatin-344 reconstituted?
Follistatin-344 ships as a lyophilised powder and is reconstituted with bacteriostatic water before use, then drawn on an insulin syringe. Use the calculator above to see the exact units to draw for your vial size and dose. This is a reference tool, not medical advice.
How should Follistatin-344 be stored?
Store lyophilized powder frozen at -20°C, stable 1+ year; refrigerated (2-8°C) stable 1-3 months; room temperature only 2-3 weeks in emergencies. Once reconstituted: Refrigerate at 2-8°C after reconstitution with bacteriostatic water; never freeze reconstituted solution. (about 28 days). This is general storage information, not medical advice.
Keep your protocol, doses, and vials in one place.
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