Peplo · Reconstitution calculator

ARA-290

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Reconstitution calculator

Vial Amount

The total peptide in the vial — the mg number on the label (e.g. 5 mg). This is your whole supply, not one dose.

Bacteriostatic Water

The sterile water you add to dissolve the powder (this is called reconstituting). More water makes small doses easier to measure.

Desired Dose

How much you take in one injection. 1 mg = 1000 mcg.

Syringe Size

Draw to 48 units

0.48 mL on a U-100 insulin syringe

A starting reference, not medical advice — see how we source this.

Half-life: Not established
Route: SubQ
Storage: Refrigerate 2–8°C, protected from light; use within 28 days; do not freeze. (~28 days)
Citations: 2 referenced

US regulatory status

Not FDA-approved; studied in early clinical settings as a research compound rather than an approved treatment.

What is ARA-290?

ARA-290, also known as cibinetide, is an 11-amino-acid non-erythropoietic peptide derived from the helix-B domain of erythropoietin. It has been studied for diabetic neuropathy and sarcoidosis-related small-fiber neuropathy through activation of the innate repair receptor.

How ARA-290 works

Selectively activates the innate repair receptor (IRR), an EPOR/CD131 (βc) heterodimer distinct from the classical erythropoietin receptor, triggering anti-inflammatory and anti-apoptotic tissue-protective signaling without stimulating red-blood-cell production. Analgesic effects have also been linked to inhibition of the capsaicin-activated TRPV1 ion channel.

What the research says

Research describes the peptide selectively activating the innate repair receptor, an EPOR/CD131 heterodimer distinct from the classical erythropoietin receptor, in the context of anti-inflammatory and tissue-protective signaling without stimulating red-blood-cell production; analgesic effects have also been linked to inhibition of the TRPV1 ion channel. One cited trial examined ARA-290 alongside metabolic control and neuropathic symptoms in patients with type 2 diabetes. Another looked at symptoms and corneal nerve fiber density in patients with sarcoidosis-associated small nerve fiber loss.

Evidence limitations

These trials represent a small body of human research.

Safety & use boundary

ARA-290 is a research compound studied in early clinical settings rather than an approved treatment. This page is educational information and is not medical advice; consult a qualified clinician before considering it.

Published protocols

Dosing figures reported in the cited research. Educational reference, not a recommendation.

injury-repair
2–4 mg, once daily
2014 study

How to reconstitute ARA-290

New to this? Here's the whole process, start to finish. Educational only — not medical advice.

What you'll need

The ARA-290 vial — a small glass vial of white, freeze-dried powder
Bacteriostatic water — the liquid you mix in (not plain sterile or tap water)
U-100 insulin syringes — the small syringes marked in units
Alcohol swabs to wipe the vial tops before you touch them with a needle
A spot in the fridge to keep it once it's mixed

Step by step

Wipe everything down
Swab the rubber top of both the peptide vial and the bacteriostatic water with an alcohol wipe. Clean tops are what keep the mixed solution good for weeks instead of days.
Draw the water
Use the calculator above to see how much bacteriostatic water to add for your vial size. Pull exactly that amount out of the water vial with a syringe.
Add it slowly
Push the needle into the peptide vial and let the water run gently down the inside glass wall — don't blast it straight onto the powder.
Let it dissolve
Swirl the vial gently or just let it sit. The powder goes into solution on its own in a minute or two. Never shake it hard.
Read your draw
Enter your vial size, the water you added, and your dose in the calculator. It shows the exact units to pull into your syringe — you never do the math yourself.
Store it cold
Cap it and keep it in the fridge. ARA-290 stays good for about 28 days once it's mixed.

Reading your dose

The calculator gives you a number like “Draw to 20 units.” Units are just the numbered marks on a U-100 insulin syringe (100 units fill 1 mL). “20 units” means pull the plunger back to the 20 mark. You don't convert milligrams to micrograms in your head — that's the whole point of the calculator. If you're brand new to injecting, learn proper technique and site rotation from a qualified clinician or nurse before your first dose; this page covers the mixing and measuring, not the injection itself.

Storing it

Before mixing: Lyophilized — refrigerate 2–8°C (up to ~2 years) or freeze at −20°C for extended storage (up to ~3 years). Once reconstituted: Refrigerate 2–8°C, protected from light; use within 28 days; do not freeze. (about 28 days). Don't freeze a reconstituted vial.

What “cycling” means

Many peptides are used in “cycles” — a stretch of daily or weekly use (“on”), then a break (“off”), rather than continuously. Cycling gives the body a rest and is a common pattern in the research. Any specific on/off lengths for a compound live in its published protocols above, drawn from the cited studies — this site shows you what the research used, it doesn't recommend a schedule for you.

Common first-timer mistakes

Shaking the vial to mix it
Swirl it gently or just let it sit. Shaking can damage delicate peptides — you want the powder to dissolve, not foam.
Using tap water or plain sterile water
Use bacteriostatic water. Plain sterile water has no preservative, so a mixed vial spoils in a day or two instead of lasting weeks.
Leaving a mixed vial on the counter
Keep reconstituted vials in the fridge. Heat and light break the peptide down faster.
Eyeballing the dose instead of measuring
Use the calculator and draw to the exact unit mark on the syringe. Guessing a tiny volume is where most measuring errors happen.
Reusing needles
Use a fresh syringe each time. A used needle is duller, hurts more, and is no longer sterile.
Mixing up mg and mcg
1 mg = 1000 mcg. The calculator keeps them straight so you don't have to — just enter your dose in the unit it asks for.

Words you'll see

Reconstitution: Mixing a freeze-dried peptide powder with liquid (bacteriostatic water) so it can be drawn into a syringe.
Bacteriostatic water: Sterile water with a tiny amount of benzyl alcohol that stops bacteria growing, so a mixed vial keeps for weeks. Not the same as plain sterile water.
U-100 insulin syringe: A small syringe marked in “units,” where 100 units = 1 mL. Peptide draws are measured in these units.
Subcutaneous (SubQ): An injection into the fatty layer just under the skin, rather than into muscle or a vein.
mcg vs mg: 1 mg = 1000 mcg. Doses are often written in micrograms (mcg); the calculator handles the conversion for you.
Lyophilized: Freeze-dried. The powder in the vial is the peptide with its water removed so it stays stable on the shelf.
Half-life: Roughly how long it takes your body to clear half of a dose — a rough sense of how long it stays active.

Common questions

What is ARA-290?

Cibinetide, an 11-amino-acid non-erythropoietic peptide derived from erythropoietin's helix-B domain; studied for diabetic neuropathy and sarcoidosis-related small-fiber neuropathy via innate repair receptor activation. Selectively activates the innate repair receptor (IRR), an EPOR/CD131 (βc) heterodimer distinct from the classical erythropoietin receptor, triggering anti-inflammatory and anti-apoptotic tissue-protective signaling without stimulating red-blood-cell production. Analgesic effects have also been linked to inhibition of the capsaicin-activated TRPV1 ion channel.

How is ARA-290 reconstituted?

ARA-290 ships as a lyophilised powder and is reconstituted with bacteriostatic water before use, then drawn on an insulin syringe. Use the calculator above to see the exact units to draw for your vial size and dose. This is a reference tool, not medical advice.

How should ARA-290 be stored?

Lyophilized — refrigerate 2–8°C (up to ~2 years) or freeze at −20°C for extended storage (up to ~3 years). Once reconstituted: Refrigerate 2–8°C, protected from light; use within 28 days; do not freeze. (about 28 days). This is general storage information, not medical advice.

Keep your protocol, doses, and vials in one place.

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